α-aiolos antibody Search Results


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Identification and enumeration of aiolos+ cells by flow cytometry
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90
Becton Dickinson alexa fluor 647-anti-aiolos antibody
Alexa Fluor 647 Anti Aiolos Antibody, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/%CE%B1-aiolos+antibody/alexa+fluor+647+anti+aiolos+antibody/10__1172_slash_jci172573-324-7-11
Average 90 stars, based on 1 article reviews
alexa fluor 647-anti-aiolos antibody - by Bioz Stars, 2026-10
90/100 stars
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90
Becton Dickinson α-aiolos (s48-791, dilution 1:50)
<t>Aiolos</t> and STAT3 physically interact and regulate Bcl6 expression. (A) Co-immunoprecipiation (Co-IP) of endogenously expressed proteins in TFH-like cells with control antibody (α-V5) or α-Ikaros, followed by immunoblot analysis (IB) with <t>α-Aiolos.</t> Shown is a representative blot of four independent immunoprecipitation experiments performed. (B) Co-IP of endogenously expressed proteins in TFH-like cells with control antibody (α-V5) or α-STAT3, followed by immunoblot analysis (IB) with α-Aiolos. Shown is a representative blot of three independent immunoprecipitation experiments performed. (C) EL4 T cells were transfected with Aiolos alone, STAT3CA alone, Aiolos and STAT3CA (increasing amounts indicated by wedge) in combination, or empty vector control. Immunoblot with anα-V5 antibody was performed to assess relative abundance of overexpressed Aiolos and STAT3CA. Following a 24 hr time period, RNA was isolated and Bcl6 expression was measured by qRT-PCR. Data were normalized to Rps18 as a control and the results are represented as fold change in expression relative to the control (ctrl.) sample (mean of n = 3 ± s.e.m.). *P< 0.05, **P< 0.01 (one-way ANOVA with Tukey multiple comparison test).
α Aiolos (S48 791, Dilution 1:50), supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/%CE%B1-aiolos+antibody/%CE%B1+aiolos++s48+791++dilution+1+50++antibody/pmc05657606-145-9-20
Average 90 stars, based on 1 article reviews
α-aiolos (s48-791, dilution 1:50) - by Bioz Stars, 2026-10
90/100 stars
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94
Bio-Techne corporation aiolos/ikzf3 antibody
<t>Aiolos</t> and STAT3 physically interact and regulate Bcl6 expression. (A) Co-immunoprecipiation (Co-IP) of endogenously expressed proteins in TFH-like cells with control antibody (α-V5) or α-Ikaros, followed by immunoblot analysis (IB) with <t>α-Aiolos.</t> Shown is a representative blot of four independent immunoprecipitation experiments performed. (B) Co-IP of endogenously expressed proteins in TFH-like cells with control antibody (α-V5) or α-STAT3, followed by immunoblot analysis (IB) with α-Aiolos. Shown is a representative blot of three independent immunoprecipitation experiments performed. (C) EL4 T cells were transfected with Aiolos alone, STAT3CA alone, Aiolos and STAT3CA (increasing amounts indicated by wedge) in combination, or empty vector control. Immunoblot with anα-V5 antibody was performed to assess relative abundance of overexpressed Aiolos and STAT3CA. Following a 24 hr time period, RNA was isolated and Bcl6 expression was measured by qRT-PCR. Data were normalized to Rps18 as a control and the results are represented as fold change in expression relative to the control (ctrl.) sample (mean of n = 3 ± s.e.m.). *P< 0.05, **P< 0.01 (one-way ANOVA with Tukey multiple comparison test).
Aiolos/Ikzf3 Antibody, supplied by Bio-Techne corporation, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/%CE%B1-aiolos+antibody/Aiolos%2FIKZF3+Antibody/bio-techne+corporation___nbp2-16938
Average 94 stars, based on 1 article reviews
aiolos/ikzf3 antibody - by Bioz Stars, 2026-10
94/100 stars
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Boster Bio Anti-Zinc finger protein Aiolos IKZF3 Antibody catalog # A01611-1. Tested in WB,ICC/IF applications. This antibody reacts with Human,Mouse.
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Boster Bio Anti-Aiolos (S386) IKZF3 Antibody catalog # A01611S386. Tested in WB applications. This antibody reacts with Human,Mouse,Rat.
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N/A
Identification and enumeration of aiolos+ cells by flow cytometry
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Image Search Results


Aiolos and STAT3 physically interact and regulate Bcl6 expression. (A) Co-immunoprecipiation (Co-IP) of endogenously expressed proteins in TFH-like cells with control antibody (α-V5) or α-Ikaros, followed by immunoblot analysis (IB) with α-Aiolos. Shown is a representative blot of four independent immunoprecipitation experiments performed. (B) Co-IP of endogenously expressed proteins in TFH-like cells with control antibody (α-V5) or α-STAT3, followed by immunoblot analysis (IB) with α-Aiolos. Shown is a representative blot of three independent immunoprecipitation experiments performed. (C) EL4 T cells were transfected with Aiolos alone, STAT3CA alone, Aiolos and STAT3CA (increasing amounts indicated by wedge) in combination, or empty vector control. Immunoblot with anα-V5 antibody was performed to assess relative abundance of overexpressed Aiolos and STAT3CA. Following a 24 hr time period, RNA was isolated and Bcl6 expression was measured by qRT-PCR. Data were normalized to Rps18 as a control and the results are represented as fold change in expression relative to the control (ctrl.) sample (mean of n = 3 ± s.e.m.). *P< 0.05, **P< 0.01 (one-way ANOVA with Tukey multiple comparison test).

Journal: Journal of immunology (Baltimore, Md. : 1950)

Article Title: Integrated STAT3 and Ikaros Zinc Finger transcription factor activities regulate Bcl-6 expression in CD4 + T helper cells

doi: 10.4049/jimmunol.1700106

Figure Lengend Snippet: Aiolos and STAT3 physically interact and regulate Bcl6 expression. (A) Co-immunoprecipiation (Co-IP) of endogenously expressed proteins in TFH-like cells with control antibody (α-V5) or α-Ikaros, followed by immunoblot analysis (IB) with α-Aiolos. Shown is a representative blot of four independent immunoprecipitation experiments performed. (B) Co-IP of endogenously expressed proteins in TFH-like cells with control antibody (α-V5) or α-STAT3, followed by immunoblot analysis (IB) with α-Aiolos. Shown is a representative blot of three independent immunoprecipitation experiments performed. (C) EL4 T cells were transfected with Aiolos alone, STAT3CA alone, Aiolos and STAT3CA (increasing amounts indicated by wedge) in combination, or empty vector control. Immunoblot with anα-V5 antibody was performed to assess relative abundance of overexpressed Aiolos and STAT3CA. Following a 24 hr time period, RNA was isolated and Bcl6 expression was measured by qRT-PCR. Data were normalized to Rps18 as a control and the results are represented as fold change in expression relative to the control (ctrl.) sample (mean of n = 3 ± s.e.m.). *P< 0.05, **P< 0.01 (one-way ANOVA with Tukey multiple comparison test).

Article Snippet: Fluorochrome-labeled α-Bcl-6 (K112.91, dilution 1:50), α-Cxcr5 (2G-8, dilution 1:50), α-Aiolos (S48-791, dilution 1:50) and α-CD4 (RM4-5, dilution 1:200) were from BD Biosciences.

Techniques: Expressing, Co-Immunoprecipitation Assay, Western Blot, Immunoprecipitation, Transfection, Plasmid Preparation, Isolation, Quantitative RT-PCR